![]() ![]() A better understanding of cell-cell or cell-niche interactions is crucial to understand the complexity of inflammatory or pathophysiological processes. Optical clearing and volumetric imaging of large tissue pieces or whole organs holds great promise for the in-depth study of cells in their natural surroundings. Tissue clearing and 3D imaging – putting immune cells into context | Journal of Cell Science | The Company of Biologists To get you started, we recommend to consult an ever growing list of reviews – if you are interested in immune cells, start with ours here: We specialize on the imaging of lymphocytes with single-cell resolution to be able to quantify them and analyse distances to other cells or vessels. To explore your cells in their „natural habitat“, plenty of tissue clearing protocols are currently used. Tissue clearing: which protocol to use and how to image? We are always interested in exploring new applications for our method – get in touch! We use confocal, widefield, as well as light sheet microscopy to image cleared organs or large tissue pieces. Recently, the Keppler lab implemented 3D imaging of organs or large tissue pieces in our workflow. Copyright: Keppler labĭuring her work in London, Selina first started imaging (confocal, 2-photon and TIRFM). Vessels in magenta, MHC-II expressing cells in cyan. Image shows a piece of white adipose tissue cleared and imaged in 3D using our EMOVI protocol (Hofmann et al., Frontiers in Immunology, 2021). ![]()
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